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Key Documents

SAB5600227

Sigma-Aldrich

Anti-His-Tag antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM146, purified immunoglobulin

Synonym(s):

10 His epitope tag, 6 His epitope tag, HHHHHH tag, Polyhistidine Tag

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM146, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

technique(s)

flow cytometry: 0.5-2 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.1—1 μg/mL
immunoprecipitation (IP): 0.5-2 μg/mL
western blot: 0.1— 1 μg/mL

isotype

IgG

NCBI accession no.

8350

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Specificity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Physical form

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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