SAB2702218

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

• Synonym(s): Mouse Anti-Hexa Histidine tag
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: GT359, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse
• concentration: 1mg/mL
• technique(s): ELISA: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: 500-3000
• isotype: IgG3
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

Biochem/physiol Actions

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable