Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
9050-94-6
MDL number:
MFCD00132235
UNSPSC Code:
23151817
NACRES:
NA.52

grade

DNA grade
for molecular biology

Quality Level

200

product line

BioReagent

form

beads

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 9-11 mL

bead diameter

40-120 μm

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

application(s)

life science and biopharma

compatibility

Cytiva

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 3

1 of 3

Sephadex® G-10 Medium

Sigma-Aldrich

G10120

Sephadex® G-10

Sephadex® G-50 Medium

Supelco

G50150

Sephadex® G-50

Superdex® 200 Prep Grade Cytiva 17-1043-01, pack of 150 mL

GE17-1043-01

Superdex® 200 Prep Grade

Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
S Kaushik et al.
Methods in molecular biology (Clifton, N.J.), 445, 227-244 (2008-04-22)
Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian cells able to selectively degrade cytosolic proteins in lysosomes. CMA is maximally activated in response to stressors such as prolonged starvation, exposure to toxic compounds, or oxidative stress. We
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Veronica M Chiu et al.
Analytical biochemistry, 418(1), 58-65 (2011-08-02)
An unstirred layer (USL) exists at the interface of solids with solutions. Thus, the particles in brain tissue preparations possess a USL as well as at the surface of a rotating disk electrode (RDE) used to measure chemical fluxes. Time
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service