S4942
SYPRO® Ruby Protein Gel Stain
Synonym(s):
SYPRO® dye, protein gel stain
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About This Item
UNSPSC Code:
41105322
NACRES:
NA.32
shelf life
≥6 mo. (when stored at room temperature and protected from light)
Quality Level
technique(s)
protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).
Caution
Protect from light.
Legal Information
SYPRO is a registered trademark of Life Technologies
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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