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Key Documents

S3504

Sigma-Aldrich

Sigmacell Cellulose

Type 20, 20 μm

Synonym(s):

Cellulose, Cellulose powder, Cotton linters

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.56

type

Type 20

Quality Level

particle size

20 μm

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

InChI key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

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Application

Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.
High purity cellulose powders for partition chromatography.

Reconstitution

For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stuart M Linton et al.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also
Okako Omadjela et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(44), 17856-17861 (2013-10-16)
Cellulose is a linear extracellular polysaccharide. It is synthesized by membrane-embedded glycosyltransferases that processively polymerize UDP-activated glucose. Polymer synthesis is coupled to membrane translocation through a channel formed by the cellulose synthase. Although eukaryotic cellulose synthases function in macromolecular complexes
Eshchar Mizrachi et al.
The New phytologist, 194(1), 54-62 (2012-04-05)
Fast-growing, short-rotation forest trees, such as Populus and Eucalyptus, produce large amounts of cellulose-rich biomass that could be utilized for bioenergy and biopolymer production. Major obstacles need to be overcome before the deployment of these genera as energy crops, including
Nathalie Lavoine et al.
Carbohydrate polymers, 90(2), 735-764 (2012-07-31)
Interest in microfibrillated cellulose (MFC) has been increasing exponentially. During the last decade, this bio-based nanomaterial was essentially used in nanocomposites for its reinforcement property. Its nano-scale dimensions and its ability to form a strong entangled nanoporous network, however, have
Rachel A Burton et al.
Current opinion in biotechnology, 26, 79-84 (2014-04-01)
Plant cell walls consist largely of cellulose, non-cellulosic polysaccharides and lignin. Concerted attempts are underway to convert wall polysaccharides from crop plant residues into renewable transport fuels and other valuable products, and to exploit the dietary benefits of cereal grain

Protocols

To standardize an enzymatic assay procedure of cellulase.

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