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Key Documents

R9379

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~70,000

Synonym(s):

RITC-Dextran

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About This Item

MDL number:
UNSPSC Code:
12171501
NACRES:
NA.56

mol wt

average mol wt ~70,000

Quality Level

extent of labeling

0.002-0.015 mol RITC per mol glucose

storage temp.

2-8°C

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General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye.

Application

Rhodamine B isothiocyanate-Dextran has been used:
  • to label A, CI-M6PR-GFP, Lamp1-CFP-expressing wild type mouse embryonic fibroblast (MEF) cells
  • in endosome/phagosome fusion assay to determine the rate of fusion of endosomes
  • as a component in poration medium to monitor Hela cell uptake

Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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