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P4170

Propidium iodide

≥94.0% (HPLC), fluorescent dye, powder

Synonym(s):

3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

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About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
25535-16-4
Molecular Weight:
668.39
NACRES:
NA.77
PubChem Substance ID:
57654524
UNSPSC Code:
41106305
EC Number:
247-081-0
MDL number:
MFCD00011921
Beilstein/REAXYS Number:
3843838
Assay:
≥94.0% (HPLC)
Form:
powder
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Product Name

Propidium iodide, ≥94.0% (HPLC)

Quality Segment

200

assay

≥94.0% (HPLC)

form

powder

mp

220-225 °C (dec.) (lit.)

storage temp.

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

General description

Propidium iodide (PI) is a fluorescent dye thatcan be used for staining nucleic acids.[1] It is impermeable in viable orintact cells. It can penetrate easily into dead, dying, or membrane-compromisedcells.[1][2] PI has the capacity to bind easily to DNA.[3]

Application

Propidiumiodide has been used: to detect cell cycle by flow cytometry and γH2AXand pH3 analysis[4],apoptosis assay in hepatic stellate cells (HSCs)[5], in stainingbuffer/solution to stain the cells for cell cycle analysis[6]

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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This Item
53705953706081845
Propidium iodide ≥94.0% (HPLC)

Sigma-Aldrich

P4170

Propidium iodide

Propidium Iodide Membrane impermeable DNA intercalator.

Sigma-Aldrich

537059

Propidium Iodide

Propidium Iodide Solution A convenient form of propidium iodide useful for flow cytometry studies.

Sigma-Aldrich

537060

Propidium Iodide Solution

Propidium iodide ≥94% (HPLC)

Sigma-Aldrich

81845

Propidium iodide

assay

≥94.0% (HPLC)

assay

-

assay

-

assay

≥94% (HPLC)

form

powder

form

solid

form

liquid

form

powder

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

220-225 °C (dec.) (lit.)

mp

-

mp

-

mp

220-225 °C (dec.) (lit.)

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pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H341

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Questions

1–5 of 5 Questions  

  1. How long can we stored PI dissolved in water?

    1 answer

    1. The solution stability of this specific product has not been determined, but solutions are quite stable. Product P4864, a 1 mg/mL prepared solution, is stored refrigerated and offers a warranty of 1 year from the date of shipment.

      Helpful?

  2. Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?

    1 answer

    1. The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.

      Helpful?

  3. What is the procedure for dissolving this product?

    1 answer

    1. Propidium iodide is soluble in water at a concentration of 1 mg/mL.

      Helpful?

  4. Is this reagent suited for viability assays at FACS?

    1 answer

    1. While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
      https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=

      Helpful?

  5. What is the method to dissolve this product?

    1 answer

    1. Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf

      Helpful?

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