Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

O1516

Sigma-Aldrich

Oil Red O solution

0.5% in propylene glycol

Synonym(s):

Solvent Red 27, Sudan Red 5B

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
1320-06-5
Molecular Weight:
408.49
UNSPSC Code:
12171500
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

0.5% in propylene glycol

technique(s)

microbe id | staining: suitable

λmax

0.40-1.00 at 506-520 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

Looking for similar products? Visit Product Comparison Guide

General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

217.4 °F

Flash Point(C)

103 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes.
Noriega-Gonzalez J E, et al.
International Journal of Morphology, 33(3) (2015)
Alyssa M Flores et al.
Nature nanotechnology, 15(2), 154-161 (2020-01-29)
Atherosclerosis is the process that underlies heart attack and stroke. A characteristic feature of the atherosclerotic plaque is the accumulation of apoptotic cells in the necrotic core. Prophagocytic antibody-based therapies are currently being explored to stimulate the phagocytic clearance of
Francesco Albanese
Canine and Feline Skin Cytology: A Comprehensive and Illustrated Guide to the Interpretation of Skin Lesions via Cytological Examination (2016)
Bingning Dong et al.
Hepatology (Baltimore, Md.), 71(5), 1559-1574 (2019-09-12)
Obesity-induced chronic inflammation is a key component in the pathogenesis of nonalcoholic fatty liver disease (NAFLD) and insulin resistance. Increased secretion of proinflammatory cytokines by macrophages in metabolic tissues promotes disease progression. In the diet-induced obesity (DIO) mouse model, activation
Jesse W Williams et al.
Nature immunology, 21(10), 1194-1204 (2020-09-09)
Early atherosclerosis depends upon responses by immune cells resident in the intimal aortic wall. Specifically, the healthy intima is thought to be populated by vascular dendritic cells (DCs) that, during hypercholesterolemia, initiate atherosclerosis by being the first to accumulate cholesterol.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service