N0766

Nile Blue A

Dye content, ≥75%, certified by the Biological Stain Commission, powder

• Synonym(s): Basic Blue 12, Nile blue sulfate
• Linear Formula: 2C₂₀H₂₀N₃O · SO₄
• CAS Number: 3625-57-8
• Molecular Weight: 732.85
• Colour Index Number: 51180
• EC Number: 222-832-5
• MDL number: MFCD00064529
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897446
• NACRES: NA.47

Properties

• product name: Nile Blue A, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• form: powder
• composition: Dye content, ≥75%
• technique(s): microbe id | staining: suitable
• color: dark green
• mp: >300 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78
• InChI: 1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
• InChI key: QIRDPEPUXNCOLD-UHFFFAOYSA-N

Description

Application

Nile Blue A has been used for the staining of eggs before transferring them to the hosts. It has been used for the staining of cells.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

Suitability

Certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves