Skip to Content
Merck
All Photos(1)

Key Documents

MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

2-8°C

General description

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Principle

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Meng-Yan Sun et al.
Oncology reports, 39(6), 2673-2680 (2018-04-06)
Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from
Shu Yan Liu et al.
Oncotarget, 7(13), 16282-16296 (2016-02-27)
Increased integrin β4 (ITGB4) level is accompanied by malignant progression of multiple carcinomas. However, selective therapeutic strategies against cancer cells expressing a high level of ITGB4 have not been reported. Here, for the first time, we report that a chiral
Senyuan Cai et al.
Bioorganic & medicinal chemistry, 27(7), 1274-1282 (2019-02-23)
Filamenting temperature-sensitive mutant Z (FtsZ) is recognized as a promising target for new antibiotics development because of its high conservatism and pivotal role in the bacteria cell division. The aromatic heterocyclic scaffold of indole is known showing merit medical functions
Meifang Li et al.
International journal of biological sciences, 15(12), 2522-2537 (2019-11-23)
Despite remarkable advancements in our understanding of breast cancer, it remains the leading cause of cancer deaths in women. Distant recurrence and metastasis is the main reason for death due to breast cancer. It is well recognized that the GATA
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service