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M3795

Sigma-Aldrich

IgM, Kappa from murine myeloma

clone TEPC 183, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Mouse IgM-κ

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

Pricing and availability is not currently available.

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

TEPC 183, monoclonal

Assay

≥90% (HPLC)

form

buffered aqueous solution

concentration

2.0-2.2 mg/mL

shipped in

dry ice

storage temp.

−20°C

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General description

Determined by the absorbance at 280 nm (E1%/280 = 11.8). Each vial contains at least 1 mg of purified myeloma protein.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens[1] IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.[2] It is secreted by peritoneal B cells.[3]

Specificity

The TEPC 183 tumor line is pristine-induced plasmacytoma-originated and carried subcutaneously in BALB/c mice. The hapten binding specificity of the TEPC 183 line has not been determined. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.

Application

IgM, Kappa from murine myeloma has been used in:
  • sandwich enzyme linked immunosorbent assay (ELISA)[4][5]
  • flow cytometry[6]
  • dot-immunobinding assay[7]

Biochem/physiol Actions

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections.[3] IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.[8] IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid.[9] IgM linked agarose resins have been tested for efficient conjugate binding.[10]

Physical form

Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    A fast and simple dot-immunobinding assay for quantification of mouse immunoglobulins in hybridoma culture supernatants
    Sulimenko T and Draber P
    Journal of Immunological Methods, 289(1-2), 89-95 (2004)
    A-O Hovden et al.
    Scandinavian journal of immunology, 62(1), 36-44 (2005-08-12)
    The aim of this study was to compare the kinetics and the magnitude of the humoral immune response to two different influenza vaccine formulations, whole and split virus vaccines. BALB/c mice were immunized intramuscularly with one or two doses (3
    Matthew P Collin et al.
    Transplantation, 79(6), 722-725 (2005-03-24)
    Graft-versus-host disease (GvHD), a life-threatening complication of bone marrow transplantation, is initiated by donor T cells reacting to recipient dendritic cells (DC). GvHD can be controlled by attenuating donor T cells, but few strategies exist to target DC, particularly resident
    T Sulimenko et al.
    Journal of immunological methods, 289(1-2), 89-95 (2004-07-15)
    Mouse monoclonal antibodies of IgG subclasses and IgM class in hybridoma culture supernatants were quantified using a dot-immunobinding assay. Immunoglobulins were bound to nitrocellulose (NC) membrane and, after blocking, the membrane was incubated with anti-mouse antibody conjugated to horseradish peroxidase
    Sequential genetic modification of the hprt locus in human ESCs combining gene targeting and recombinase-mediated cassette exchange
    Di Domenico AI, et al.
    Cloning and Stem Cells, 10(2), 217-230 (2008)

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