Skip to Content
Merck
All Photos(1)

Key Documents

I8015

Sigma-Aldrich

IgG from rat serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Synonym(s):

Rat IgG

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

unconjugated

Quality Level

grade

technical grade

Assay

≥80% (SDS-PAGE)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

IgG (immunoglobulin G) antibody is enriched in mother′s milk. IgG is a major class of immunoglobulin. It contributes to 10−20% of plasma protein and is regarded as one of the most abundant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. The chains are interlinked with a disulfide bond. Both the L and the H chains consist of a variable domain at the N-terminal. The C-terminal bears′ constant domains: three in H chain with a hinge region and one in the L chain. The variable region of IgG antibody is specific to antigens and is highly conserved.

Application

IgG from rat serum has been used:
  • To block programmed death ligand 1.
  • As a control antibody to study the role of integrin b1 in spindle morphology
  • In FACS (flow cytometry) staining
  • ELISA (enzyme linked immunosorbent assay)

IgG from rat serum was used to block non-specific staining during FACS of peritoneal cells isolated from BALB/c mice. It was used as positive control in VDR staining of male and female breast tumor cells. IgG from rat serum was injected in control mice in tumor transplantation studies.

Biochem/physiol Actions

IgG antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain
Ishihara S, et al.
Febs Letters, 587(6), 732-736 (2013)
A Coronin 1--Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
Lang MJ, et al.
Journal of Immunology, ji1700438-ji1700438 (2017)
M Boesen et al.
Clinical and experimental immunology, 121(2), 210-215 (2000-08-10)
An attempt was made to identify the selection pressures put upon a growing tumour by CD8+ T cells. To this end tumours induced with 3-methylcholanthrene in T cell-deficient nude mice and in congenic T cell-competent nu/+ mice were transplanted to
Designing Antibodies, 216-216 (2012)
The Immunoglobulins: Structure and Function (1998)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service