Skip to Content
Merck
All Photos(1)

Key Documents

I6383

Sigma-Aldrich

Insulin Chain B Oxidized from bovine pancreas

≥80% (HPLC), powder

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C157H232N40O47S2
CAS Number:
Molecular Weight:
3495.89
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

Pricing and availability is not currently available.

sterility

non-sterile

Quality Level

Assay

≥80% (HPLC)

form

powder

mol wt

3496 Da by calculation (Average Mass)

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

InChI

1S/C157H232N40O47S2/c1-79(2)57-104(181-130(211)86(15)172-136(217)103(50-53-125(209)210)180-152(233)127(84(11)12)194-148(229)107(60-82(7)8)184-145(226)113(67-95-70-165-78-171-95)189-150(231)115(74-198)176-123(206)73-169-133(214)116(75-245(239,240)241)191-140(221)105(58-80(3)4)182-144(225)112(66-94-69-164-77-170-94)188-138(219)102(48-51-119(160)202)178-146(227)114(68-120(161)203)190-153(234)126(83(9)10)193-131(212)98(159)61-89-31-21-18-22-32-89)139(220)185-110(64-92-40-44-96(200)45-41-92)142(223)183-106(59-81(5)6)147(228)195-128(85(13)14)154(235)192-117(76-246(242,243)244)134(215)168-71-121(204)174-101(49-52-124(207)208)137(218)177-99(38-29-55-166-157(162)163)132(213)167-72-122(205)175-108(62-90-33-23-19-24-34-90)141(222)186-109(63-91-35-25-20-26-36-91)143(224)187-111(65-93-42-46-97(201)47-43-93)149(230)196-129(88(17)199)155(236)197-56-30-39-118(197)151(232)179-100(37-27-28-54-158)135(216)173-87(16)156(237)238/h18-26,31-36,40-47,69-70,77-88,98-118,126-129,198-201H,27-30,37-39,48-68,71-76,158-159H2,1-17H3,(H2,160,202)(H2,161,203)(H,164,170)(H,165,171)(H,167,213)(H,168,215)(H,169,214)(H,172,217)(H,173,216)(H,174,204)(H,175,205)(H,176,206)(H,177,218)(H,178,227)(H,179,232)(H,180,233)(H,181,211)(H,182,225)(H,183,223)(H,184,226)(H,185,220)(H,186,222)(H,187,224)(H,188,219)(H,189,231)(H,190,234)(H,191,221)(H,192,235)(H,193,212)(H,194,229)(H,195,228)(H,196,230)(H,207,208)(H,209,210)(H,237,238)(H4,162,163,166)(H,239,240,241)(H,242,243,244)/t86-,87-,88+,98-,99-,100-,101-,102-,103-,104-,105-,106-,107-,108-,109-,110-,111-,112-,113-,114-,115-,116-,117-,118-,126-,127-,128-,129-/m0/s1

InChI key

CZJQESISDHGFTC-DQCCOYHDSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
I1764I1633I5500
Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

powder

form

-

form

powder

form

powder

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

Gene Information

cow ... INS(280829)

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Amino Acid Sequence

Phe-Val-Asn-Gln-His-Leu-Cys(SO3H)-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys(SO3H)-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala

General description

Insulin is made up of two polypeptide chains (A and B) linked together by two disulfide linkages. Chain A and B are composed of 21 amino acids and 30 amino acids, respectively.[1]

Application

Insulin Chain B Oxidized from bovine pancreas has been used:
  • as a matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) calibration standard[2][3]
  • in electrochemical impedance spectroscopy and quartz crystal nanobalance (EQCN) studies of insulin adsorption on platinum[1]
  • as an internal standard to quantify insulin chain B residue in protein samples[4]

Biochem/physiol Actions

Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids. It inhibits the breakdown of glycogen, protein, and fat. The β chain is a substrate for carboxypeptidase Y.

Preparation Note

Prepared by modification of Sanger, F., et al.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Need A Sample COA?

This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

L He et al.
Journal of mass spectrometry : JMS, 34(9), 909-914 (1999-09-24)
Matrix-assisted laser desorption/ionization (MALDI) mass spectra were obtained from single particles injected directly into a time-of-flight mass spectrometer. Aerosol particles were generated at atmospheric pressure using a piezoelectric single-particle generator or a pneumatic nebulizer and introduced into the mass spectrometer
Johansen, J.T., et al.
Carlsberg Research Communications, 41, 1-1 (1976)
Electrochemical impedance spectroscopy and quartz crystal nanobalance (EQCN) studies of insulin adsorption on Pt
Wright JEI, et al.
Journal of Electroanalytical Chemistry, 564, 185-197 (2004)
Epitope mapping by a combination of epitope excision and MALDI-MS.
C E Parker et al.
Methods in molecular biology (Clifton, N.J.), 146, 185-201 (2000-08-19)
Carol E Parker et al.
Molecular biotechnology, 20(1), 49-62 (2002-03-06)
Proteolytic digestion of proteins bound to immobilized antibodies, combined with matrix assisted laser desorption (MALDI) mass spectrometric identification of the affinity-bound peptides, can be a powerful technique for epitope determination. Binding of the protein to the antibody is done while

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service