I5904

Iberiotoxin, recombinant from Mesobuthus tamulus

≥98% (HPLC), recombinant, expressed in E. coli

• Synonym(s): IbTX
• Empirical Formula (Hill Notation): C₁₇₉H₂₇₆N₅₀O₅₆S₇
• Molecular Weight: 4248.86
• UNSPSC Code: 12352200
• PubChem Substance ID: 24896080
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• Assay: ≥98% (HPLC)
• storage temp.: −20°C

Description

Amino Acid Sequence

Glp-Phe-Thr-Asp-Val-Asp-Cys-Ser-Val-Ser-Lys-Glu-Cys-Trp-Ser-Val-Cys-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys-Met-Gly-Lys-Lys-Cys-Arg-Cys-Tyr-Gln [Disulfide Bridges: 7−28; 13−33; 17−35]

Application

Iberiotoxin, recombinant from Mesobuthus tamulus has been used as an inhibitor of large-conductance calcium-activated K⁺ channels to study its effect on the NLRP3 (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3) inflammasome activation in bone marrow-derived macrophages (BMDMs). It has also been used to block Ca²⁺- activated K⁺ channels to determine interleukin(IL)-1β in BMDCs following inflammasome activation.

Biochem/physiol Actions

The single chain peptide iberiotoxin (IbTX) is a selective and reversible inhibitor of high-conductance calcium-activated potassium channels (P_K,Ca). It occurs naturally in the venom of the scorpion Buthus tamulus. IbTX also modulates the binding of charybdotoxin (CbTX) to smooth muscle sarcolemmal membranes in a non-competitive manner. IbTX is similar in size to CbTX and shares considerable sequence homology with CbTX. However, IbTX differs in its overall charge in having one fewer basic and four more acidic residues than CbTX.

Exhibits the same activity as the natural form of the toxin

Features and Benefits

This compound is featured on the Potassium Channels page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Preparation Note

Soluble in aqueous buffers. Stock solutions of 1 μM can be aliquoted and stored at -20 °C for up to 3 months.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330
• Precautionary Statements: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves