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Key Documents

H8629

Sigma-Aldrich

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

Synonym(s):

HK/G6PD

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About This Item

MDL number:
MFCD00131322
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

200

glucose-6-phosphate dehydrogenase activity

≥75 units/mg protein (biuret)

hexokinase activity

≥65 units/mg protein

purified by

crystallization

storage temp.

−20°C

General description

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.[1]
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Application

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s.[1] It has also been used for the determination of glucose and ATP.[2]

Biochem/physiol Actions

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.[3]

Packaging

Package sizes are based on hexokinase units.

Components

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Unit Definition

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000319468
0000296377
0000264626
SLCM7400
SLCK7011

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d-Glucose: Determination with Hexokinase and Glucose-6-phosphate Dehydrogenase
Slein MW
Methods of Enzymatic Analysis, 8(12), 117-130 (1965)
Laura Prioretti et al.
Journal of phycology, 52(6), 1094-1102 (2016-10-25)
The sulfate facilitation hypothesis suggests that changes in ocean sulfate concentration influenced the rise to dominance of phytoplankton species of the red lineage. The mechanistic reasons for this phenomenon are not yet understood. We started to address this question by
Fetal bovine serum contains biologically available ATP
Vultaggio-Poma V, et al.
Purinergic Signaling, 20(1), 83-89 (2024)
Effect of pH on the stability of hexokinase and glucose 6-phosphate dehydrogenase
Souza M A, et al.
Applied Biochemistry and Biotechnology, 265-272 (2002)
Samantha L Barlow et al.
The Journal of experimental biology, 220(Pt 3), 414-424 (2017-02-06)
Atlantic cod are a commercially important species believed to be threatened by warming seas near their southern, equatorward upper thermal edge of distribution. Limitations to circulatory O

Questions

1–6 of 6 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the stability of a stock solution of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?

    1 answer

    1. Stock solutions prepared in water or 10 mM potassium phosphate buffer pH 8.0 lose approximately 1% activity per month when stored at 4°C.

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  3. What units are the package sizes of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, based upon?

    1 answer

    1. This product is packaged on the basis of hexokinase units.

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  4. Can Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be used for the determination of glucose?

    1 answer

    1. A method for the determination of glucose using hexokinase and glucose 6-phosphate dehydrogenase is described in "Methods of Enzymatic Analysis" 3rd ed., Vol. VI, p. 163.

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  5. Does Sigma offer any other product which can be used in place of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?

    1 answer

    1. Yes. We offer Product No. G3293 (Glucose Assay Reagent), which is a component of the GAHK20 (Glucose Assay Kit).

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  6. How should Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be reconstituted?

    1 answer

    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it in 250 mM glycylglycine buffer, pH 7.4.

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