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H7291

Sigma-Aldrich

Interleukin-4 human

IL-4, potency:, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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About This Item

MDL number:
MFCD00151705
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.6 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 14-19 kDa (glycosylated)

packaging

pkg of 1 mg
pkg of 10 μg
pkg of 100 μg

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

P05112

storage temp.

−20°C

Gene Information

human ... IL4(3565)

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General description

Interleukin-4 (IL-4) is a pleiotropic cytokine and a complex glycoprotein mapped to human chromosome 5q31.1. It is produced by mast cells, basophils and a subset of activated T cells.

Biochem/physiol Actions

Interleukin-4 (IL-4) interacts with interleukin 13 and is an interleukin-4 receptor ligand. It regulates IgE and IgG1 production by B cells. IL-4 favors glial and neuronal differentiation and regulates differentiation of naive cluster of differentiation CD4+ T cells to the T helper type 2 (TH2) cells. Polymorphism in the IL-4 gene is implicated in immune and inflammatory disorders. In cerebral infarction, IL-4 levels are elevated.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Interleukin-4 (IL-4), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity is determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Muscle as an endocrine organ
Muscle and Exercise Physiology, 13(4) (2019)
IL-4: role in disease and regulation of production.
P Choi et al.
Clinical and experimental immunology, 113(3), 317-319 (1998-09-16)
Neurogenesis, Inflammation, and Mental Health
Inflammation and Immunity in Depression, 13(4) (2018)
Robert Y L Zee et al.
Human molecular genetics, 13(4), 389-396 (2003-12-19)
Candidate gene polymorphisms related to inflammation, thrombosis and lipid metabolism have been implicated in the development of ischemic stroke. Using DNA samples collected at baseline in a prospective cohort of 14 916 initially healthy American men, we genotyped 92 polymorphisms
Interleukin 4 Receptor
Muscle and Exercise Physiology, 13(4) (1998)
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