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H1015

25-Hydroxycholesterol

≥98%

Synonym(s):

5-Cholestene-3β,25-diol

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About This Item

Empirical Formula (Hill Notation):
C27H46O2
CAS Number:
Molecular Weight:
402.65
NACRES:
NA.77
PubChem Substance ID:
eCl@ss:
39023139
UNSPSC Code:
41141804
MDL number:
Beilstein/REAXYS Number:
3161259
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biological source

synthetic (organic)

Quality Segment

assay

≥98%

form

powder

shipped in

ambient

storage temp.

room temp

SMILES string

[H][C@@]12[C@]([C@](CC[C@H](O)C3)(C)C3=CC2)([H])CC[C@@]4(C)[C@@]1([H])CC[C@]4([H])[C@]([H])(C)CCCC(C)(O)C

InChI

1S/C27H46O2/c1-18(7-6-14-25(2,3)29)22-10-11-23-21-9-8-19-17-20(28)12-15-26(19,4)24(21)13-16-27(22,23)5/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t18-,20+,21+,22-,23+,24+,26+,27-/m1/s1

InChI key

INBGSXNNRGWLJU-ZHHJOTBYSA-N

Application

25-Hydroxycholesterol was used to treat mouse neuroblastoma cells to activate the ABCA1 signaling[1] and in studies related to fatty acid metabolism.[2][3]

Biochem/physiol Actions

Suppresses the cleavage of sterol regulatory element binding proteins (SREBPs). 25-Hydroxycholesterol induces apoptosis through down-regulation of Bcl-2 expression and activation of caspases.
25-Hydroxycholesterol is a side-chain oxysterol that reportedly triggers activation of a number of cholesterol molecules. This triggers their movement from cell membrane to the endoplasmic reticulum (ER).[4] 25-Hydroxycholesterol affects the immune system and has a key role in the pathogenesis of atherosclerosis.

Preparation Note

A stock solution of 25-Hydroxycholesterol may be prepared in ethanol and dissolved in cell culture medium to give a final concentration of 1 mg/ml.

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This Item
H9384H5884D6128
biological source

synthetic (organic)

biological source

-

biological source

-

biological source

synthetic (organic)

assay

≥98%

assay

≥98%

assay

≥98% (TLC)

assay

≥95%

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

form

powder

form

powder

form

powder

form

powder

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the solubility of 25-Hydroxycholesterol, H1015 (CAS number: 2140-46-7), and what are the storage conditions for the prepared solution?

    1 answer
    1. The solubility of 25-Hydroxycholesterol, H1015 (CAS number: 2140-46-7), is up to 20 mM in ethanol. The recommended storage for prepared solutions is at -20°C, and they should be used within 1 month of preparation.

      Helpful?

  4. What is the solubility and solution storage conditions of (2-Hydroxypropyl)-β-cyclodextrin

    1 answer
    1. (2-Hydroxypropyl)-β-cyclodextrin is soluble in water up to 100 mM, and the recommended storage conditions for the prepared solution are at -20°C. It is advisable to use the solution within 1 month once prepared.

      Helpful?

  5. 안녕하세요. Stock 농도는 어떻게 만들어야할까요?

    1 answer
    1. This product is soluble at 8 mg/mL in ethanol. The stock solution may then be diluted further with culture medium or workiing buffer.

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