GE29-0485-65

HiTrap^® TALON^® Crude

Cytiva 29-0485-65, pack of 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: TALON^® Superflow Cobalt-IMAC
• packaging: pack of 1 mL
• manufacturer/tradename: Cytiva 29-0485-65
• availability: not available in North America
• parameter: 1 ml/min - 4 ml/min flow rate; 3 bar
• column I.D.: 7 mm
• matrix: 6% cross-linked agarose
• particle size: 60-160 μm
• cleaning: 2-14(Co²⁺-stripped medium)
• working range: 3-12(Co2+-stripped medium)
• capacity: ≥20 mg binding capacity (histidine-tagged protein)
• storage temp.: 2-8°C

Description

General description

HiTrap TALON crude 1 ml and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment. TALON Superflow^™ is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

HiTrap TALON crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON Superflow beads. Prior to loading samples on HiTrap TALON crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

Features and Benefits

• Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
• Suitable for IMAC purification of histidine-tagged proteins when Ni²⁺ is not the optimal choice of metal ion.
• Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
• Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
• HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co²⁺ recharging.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Superflow is a trademark of Sterogene Bioseparations Inc.

Talon is a registered trademark of Clontech

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids