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GE17-0921-08

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-08, pack of 100 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Pricing and availability is not currently available.

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-0921-08

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

suitability

suitable for bioprocess medium

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This Item
GE17-5318-01GE17-0921-07GE17-5318-06
matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

capacity

~40 mg binding capacity(histidine-tagged protein)

capacity

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

capacity

~40 mg binding capacity(histidine-tagged protein)

packaging

pack of 100 mL

packaging

pack of 25 mL

packaging

pack of 25 mL

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-0921-08

manufacturer/tradename

Cytiva 17-5318-01

manufacturer/tradename

Cytiva 17-0921-07

manufacturer/tradename

Cytiva 17-5318-06

average diameter

90 μm

average diameter

90 μm

average diameter

90 μm

average diameter

90 μm

General description

IMAC Sepharose 6 Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

IMAC Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.

IMAC Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


  • Certificates of Analysis (COA)

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    Articles

    Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

    Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

    Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

    Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

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    Protocols

    This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

    This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service