GE17-0404-01

HiTrap^® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

• Synonym(s): Protein purification column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein G lacking albumin-binding region
• form: prepacked column
• crosslinking: cross-linked
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-0404-01
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: <4 mL/min flow rate; 42 psi
• technique(s): liquid chromatography (LC): suitable
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: (cross-linked agarose, spherical)
• matrix active group: Protein G functional group
• avg. part. size: 34 μm
• cleaning: 2-9
• working range: 3-9
• capacity: >1 mg binding capacity (lgG/column); 25 mg binding capacity(human IgG/column)
• separation technique: affinity
• storage temp.: 2-8°C

Description

General description

HiTrap^® Protein G HP columns are prepacked with Protein G Sepharose^™ High Performance for fast, easy separations of IgG from different species and subclasses.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

• High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
• 34 μm bead size for high performance, high-resolution purifications.
• Binds broad range of different species and subclasses of IgG.
• Simple and proven method giving reproducible results.
• Designed for use with syringe, peristalic pump, or chromatography system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids