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D1538

Sigma-Aldrich

Piperazine diacrylamide

For acrylamide gel electrophoresis

Synonym(s):

1,4-Bis(acryloyl)piperazine, 1,4-Di(acryloyl)piperazine, N,N′-Bis(acryloyl)piperazine, BAP, PDA, Piperazine diacrylamide

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About This Item

Empirical Formula (Hill Notation):
C10H14N2O2
CAS Number:
6342-17-2
Molecular Weight:
194.23
Beilstein:
745431
MDL number:
MFCD00077661
UNSPSC Code:
12352200
PubChem Substance ID:
24893477
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

mp

91.5-93.5 °C (lit.)

storage temp.

room temp

SMILES string

C=CC(=O)N1CCN(CC1)C(=O)C=C

InChI

1S/C10H14N2O2/c1-3-9(13)11-5-7-12(8-6-11)10(14)4-2/h3-4H,1-2,5-8H2

InChI key

YERHJBPPDGHCRJ-UHFFFAOYSA-N

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General description

1,4-Bis(acryloyl)piperazine is a reagent that can be substituted for bis-acrylamide in the preparation of polyacrylamide gels.

Application

Suitable for preparation of gels for PAGE, IEF, 2D electrophoresis and protein sequencing.

Features and Benefits

  • increases the gel strength and resolution
  • reduces background for silver background
  • can be substituted for bisacrylamide without changing polymerization protocol

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Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G Artoni et al.
Analytical biochemistry, 137(2), 420-428 (1984-03-01)
The properties of gels prepared either from acryloyl-morpholine (ACM) or from its mixtures with acrylamide and crosslinked either with bisacrylylpiperazine or with methylenebisacrylamide have been described. ACM-containing gels are compatible with organic solvents. If polymerized in water and dried, they
Su-Hyung Hong et al.
Biomarker insights, 1, 175-183 (2008-09-05)
Pancreatic cancer has a poor prognosis, in part due to lack of early detection. The identification of circulating tumor antigens or their related autoantibodies provides a means for early cancer diagnosis. We have used a proteomic approach to identify proteins
Ronaldo de Carvalho Augusto et al.
PLoS neglected tropical diseases, 11(7), e0005789-e0005789 (2017-07-29)
Schistosomiasis has been reported in 78 endemic countries and affects 240 million people worldwide. The digenetic parasite Schistosoma mansoni needs fresh water to compete its life cycle. There, it is susceptible to soluble compounds that can affect directly and/or indirectly
Arupa Ganguly
Human mutation, 19(4), 334-342 (2002-04-05)
Conformation-sensitive gel electrophoresis (CSGE) was developed as a method of heteroduplex analysis to screen large multi-exon genes for sequence variation. The novelty of the method was in the use of a non-proprietary acrylamide gel matrix that used 1,4-bis (acrolyl) piperazine
Guillaume Tetreau et al.
Frontiers in immunology, 8, 1249-1249 (2017-10-21)
Detection of pathogens by all living organisms is the primary step needed to implement a coherent and efficient immune response. This implies a mediation by different soluble and/or membrane-anchored proteins related to innate immune receptors called PRRs (pattern-recognition receptors) to
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