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CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

Synonym(s):

Acid Phosphatase Enzyme Activity Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
42010102
NACRES:
NA.84

Quality Level

usage

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Kit Components Only

Product No.
Description

  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets

  • Citrate buffer solution, 0.09 M pH 4.8 100 mL

  • p-Nitrophenol Standard solution 10 mM 1 mL

  • Acid phosphatase control enzyme .2 mL

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaogang Xu et al.
Frontiers in immunology, 9, 2321-2321 (2018-11-22)
Glycyrrhizin (GL), a triterpenoid glycoside, serves important functions in various biological activities, including antiviral and antitumor immune responses. However, the anti-inflammatory effects of GL on Salmonella enterica serovar Typhimurium (ST)-induced injury in mice and the mechanisms underlying the protection of
Lan Xiao et al.
OncoTargets and therapy, 9, 1105-1114 (2016-04-14)
To identify the role of YAP in cisplatin resistance in human ovarian cancer cells and in the regulation of autophagy in these cancer cells. The cisplatin-sensitive OV2008 parental cell line and its cisplatin-resistant variant C13K were cultured. RNA interference was
Qinghe Song et al.
PloS one, 10(3), e0120790-e0120790 (2015-03-27)
The Yes-associated protein (YAP), a transcriptional coactivator inactivated by the Hippo tumor suppressor pathway, functions as an oncoprotein in a variety of cancers. However, its contribution to breast cancer remains controversial. This study investigated the role of YAP in breast
Archana Vaithilingam et al.
BMC cell biology, 14, 35-35 (2013-08-14)
Endolysosomes play a key role in maintaining the homeostasis of the cell. They are made of a complex set of proteins that degrade lipids, proteins and sugars. Studies involving endolysosome contribution to cellular functions such as MHC class I and
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic

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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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