Skip to Content
Merck
All Photos(1)

Key Documents

LL24

90073001, human lung, Fibroblast

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

Pricing and availability is not currently available.

product name

LL24, 90073001

biological source

human lung

growth mode

Adherent

karyotype

2n = 46; diploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human diploid lung

Cell Line Description

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

Application

Tumourigenicity studies

Culture Medium

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, a further 20 population doublings expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service