Depending on the nature of the sample, regeneration is normally preformed by washing with a high ionic strength buffer (1-2 M NaCl) and/or increasing pH, followed by re-equilibrating in binding buffer.
C50120
CM Sephadex® C-50
Synonym(s):
CM Sephadex®, Carboxymethyl Sephadex®
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About This Item
Pricing and availability is not currently available.
Recommended Products
form
powder
Quality Level
technique(s)
activity assay: suitable
bead size
40-125 μm (dry)
pore size
~200,000 Da exclusion limit
operating pH
6-10
capacity
4.0-5.0 meq/g ion exchange capacity
compatibility
mode of use weak cation exchange chromatography
General description
C50120-100G′s updated product number is GE17-0220-01
Application
Sephadex C-50 is used for cation exchange media, separation media, resins, ion exchange chromatography, protein chromatography and affinity chromatography. Sephadex® C-50 has been used to study biochemical characteristics of cobra venom and effects of vitamins on lactoperoxidase enzyme activity.
Legal Information
Sephadex is a registered trademark of Cytiva
replaced by
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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How do I regenerate CM Sephadex® C-50, Product C50120?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I prepare CM Sephadex® C-50, Product C50120?
1 answer-
Weigh out the required amount of dry powder and suspend it in binding buffer. In general, 1 gram of dry powder CM Sephadex C-50 will give about 30 mL final volume, when swollen in saline buffer. Swelling should be done at the pH at which the resin is going to be used. Complete swelling takes 1-2 days at room temperature, or 2 hours at 100°C over boiling water.
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What molecular weight range is CM Sephadex® C-50, Product C50120, suitable for?
1 answer-
Sephadex C-50 is suitable for larger biomolecules such as proteins in the molecular weight range of 30 to 100 kDa.
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