C2331

Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse

clone C6G9, ascites fluid

• MDL number: MFCD00162210
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: C6G9, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using human colon carcinoma tissue; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P13688
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CEACAM5(1048)

Description

General description

Carcinoembryonic Antigen (CEA) is a member of the immunoglobulin superfamily that regulates cellular signaling and adhesion. CEA inhibits cell differentiation and subsequently facilitates tumor growth . Thus, CEA has been implicated in various cancers, including colorectal, lung and breast tumors
Monoclonal Anti-Human Carcinoembryonic Antigen (CEA) reacts specifically with human CEA (CD66e, 180 kDa) from different types of malignant tissues. The antibody detects an epitope which is resistant to 30 minute oxidation by 1% sodium periodate solution in formalin-fixed, paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes enhances immunohistochemical staining with the antibody.The product reacts with medullary thyroid cancers and also associates with cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. Additionally, the product reacts weakly with normal colon mucosa and occasionally reacts with bile canalicular and pancreatic acinar cells. However, the antibody does not react with other tested tissue such as term placenta, nor does it bind to non-specific cross-reacting antigen (NCA) in granulocytes.

Carcinoembryonic Antigen (CEA) is located on human chromosome 19q13.2.

Specificity

The antibody reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissue including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested tissue including term placenta. There is no cross-reactivity with non-specific cross-reacting antigen (NCA) present in granulocytes, using immunoblotting, flow cytometry and immunohistochemical techniques. The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed and paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes improves immunohistochemical staining with the antibody.

Immunogen

carcinoembryonic antigen (CEA) isolated from a human colon adenocarcinoma cell line.

Application

Monoclonal Anti-Carcinoembryonic Antigen antibody is suitable for use in immunohistochemistry (1:8,000 using formalin-fixed, paraffin-embedded sections of human colon carcinoma tissue).

Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse has been used in western blot analysis and surface plasmon resonance (SPR) based immunoassay.

Biochem/physiol Actions

Carcinoembryonic Antigen (CEA) inhibits tumor growth in colon, hepatoma and prostate cancers.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable