Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

B9673

Sigma-Aldrich

1-Bromo-3-chloropropane

for isolation of RNA

Synonym(s):

1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Linear Formula:
Cl(CH2)3Br
CAS Number:
109-70-6
Molecular Weight:
157.44
Beilstein:
605278
EC Number:
203-697-1
MDL number:
MFCD00000998
UNSPSC Code:
41105515
PubChem Substance ID:
24892149
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

Assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

ClCCCBr

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
  • feline teeth
  • mice brain and liver tissues
  • human ectocervical epithelial cells
  • placental samples
  • human mesenchymal stromal cells (hMSCs)

Features and Benefits

  • less toxic alternative to chloroform
  • compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

related product

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Statements

H302,H331,H335,H341,H412

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins
Bischoff SR, et al.
Testing, 8(1), e55345-e55345 (2013)
Isolation of total RNA from transgenic mouse melanoma subsets using fluorescence-activated cell sorting
Microarray methods for drug discovery, 27-44 (2010)
Neuroprotection Induced by Energy and Protein-Energy Undernutrition Is Phase-Dependent After Focal Cerebral Ischemia in Mice
de Carvalho TS, et al.
Translational Stroke Research, 1-12 (2019)
ALCAM (CD166) as a gene expression marker for human mesenchymal stromal cell characterisation
Brinkhof, et al.
Gene: X, 5, 100031-100031 (2020)
View All Related Papers

Protocols

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service