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78830

Sigma-Aldrich

Phenylmethanesulfonyl fluoride

≥99.0% (T)

Synonym(s):

α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride

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About This Item

Empirical Formula (Hill Notation):
C7H7FO2S
CAS Number:
Molecular Weight:
174.19
Beilstein:
2088311
EC Number:
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.77

Pricing and availability is not currently available.

biological source

synthetic

Quality Level

Assay

≥99.0% (T)

form

powder or crystals

impurities

≤0.1% water

mp

92-95 °C

solubility

anhydrous isopropanol: soluble 35 mg/mL, clear to faintly hazy, colorless to faintly yellow (Heating required)

storage temp.

room temp

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

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1 of 4

This Item
HPA007571HPA007316SAB4300232
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

immunofluorescence: 1:50-1:200, western blot: 1:500-1:2000, immunohistochemistry: 1:50-1:200

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:500-1:1000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:200-1:500

technique(s)

western blot: 1:500-1:1000

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Application

Phenylmethanesulfonyl fluoride has been used as a component in radioimmunoprecipitation buffer for western blot analysis.[1][2] It has also been used as a component in the RAW264.7 cell lysis buffer for western blotting.[3]

Biochem/physiol Actions

Phenylmethanesulfonyl fluoride has the ability to enhance the stability of plasma lipidome in lipidomic and metabolomic analysis.[4] This serine protease inhibitor reduces the naloxone-precipitated withdrawal jumping behavior in morphine-dependent mice.[5]

Other Notes

Specific trypsin and chymotrypsin inhibitor.[6] Less toxic substitute for diisopropylfluorophosphate. Potentiates the effects of ischemia on brain mitochondria through its inhibition of phospholipase C.[7]
Specific trypsin and chymotrypsin inhibitor[6]; Less toxic substitute for diisopropylfluorophosphate. Potentiates the effects of ischemia on brain mitochondria through its inhibition of phospholipase C.[7]

Pictograms

Skull and crossbonesCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Skin Corr. 1B

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

  • Certificates of Analysis (COA)

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    beta-catenin dosage is a critical determinant of tracheal basal cell fate determination
    Brechbuhl H M, et al.
    The American Journal of Pathology, 179(1), 367-379 (2011)
    D.E. Fahrney et al.
    Journal of the American Chemical Society, 85, 997-997 (1963)
    Q S Wang et al.
    Acta pharmacologica Sinica, 22(3), 249-252 (2001-12-18)
    To study the effect of phenylmethylsulfonyl fluoride (PMSF), a phospholipase C inhibitor, on ischemic injury to brain mitochondria in rats. The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria were measured. The effect of phenylmethylsulfonyl fluoride was
    Phenylmethanesulfonyl fluoride pretreatment stabilizes plasma lipidome in lipidomic and metabolomic analysis
    Wang X, et al.
    Analytica Chimica Acta, 893, 77-83 (2015)
    Cellular bioenergetics changes in magnocellular neurons may affect copeptin expression in the late phase of sepsis
    Oliveira-Pelegrin G R, et al.
    Journal of Neuroimmunology, 267(1-2), 28-34 (2014)

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