57620
Inulinase from Aspergillus niger
lyophilized, powder, brown-gray, ~25 U/mg
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About This Item
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biological source
Aspergillus niger
Quality Level
form
powder
quality
lyophilized
specific activity
~25 U/mg
color
brown-gray
storage temp.
2-8°C
General description
Inulinases are classified as exoinulinases and endoinulinases.
Application
Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.
Biochem/physiol Actions
Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.
Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.
Unit Definition
1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin
Other Notes
Characterization
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Biotechnology and Applied Biochemistry, 11, 105-105 (1989)
Bioprocess and biosystems engineering, 35(3), 383-388 (2011-08-13)
An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of
Journal of applied microbiology, 111(6), 1371-1380 (2011-09-08)
The aim of this study is to improve exoinulinase production by expression of a cloned exoinulinase gene inuA1 (GenBank accession no. JF961344) from Penicillium janthinellum strain B01 in Pichia pastoris. A full-length cDNA of exoinulinase gene (inuA1) was cloned from
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(7), 1032-1039 (2011-10-25)
Ethanol fermentation from Jerusalem artichoke tubers by recombinant Saccharomyces cerevisiae strains expressing the inulinase gene (inu) from Kluyveromyces marxianus was investigated. The inu native and pgk promoters were used to drive the expression of the inu gene, and the inulinase
Letters in applied microbiology, 55(3), 195-201 (2012-06-26)
A newly isolated strain of Rhizoctonia ssp. was used for the production of extracellular inulinase. Previously, the qualitative effects of some carbon and nitrogen sources from fermentative media and the physicochemical parameters for growth were established by Plackett-Burman analysis, and
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