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32857

Sigma-Aldrich

Wright Stain solution

according to Wright

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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About This Item

MDL number:
MFCD00005038
UNSPSC Code:
12171500
PubChem Substance ID:
329754363
NACRES:
MA.02

form

liquid

Quality Level

200

quality

according to Wright

impurities

methanol

density

0.8 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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General description

Wright′s stain solution is commercially available and is generally used in hematology. The procedure of making it includes heating methylene blue with sodium bicarbonate, followed by combining the mixture with eosin. It is an alkaline solution; hence its concentration is continuously changing, owing to the progressive oxidizing action of the alkali, therefore Giemsa′s stain is added to preserve the azurophilic properties of the polychrome dyes.

Application

Wright stain solution was used during the exudate analysis of white blood cell concentration.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

51.8 °F

Flash Point(C)

11 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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A simplified Wright's stain technique for routine avian blood smear staining.
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