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19924

Sigma-Aldrich

Pyroglutamate Aminopeptidase from Pyrococcus furiosus, recombinant from E. coli

7-13 mU (per vial)

Synonym(s):

Pyrase, Pyroglutamyl-Peptidase I, Pyrrolidone Carboxyl Peptidase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

lyophilized

specific activity

7-13 mU (per vial)

mol wt

Mr ~28000

storage temp.

−20°C

Packaging

package of 0.01 Unit

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol Pyroglutamate-p-nitroanilide per minute at pH 7.0 and 37 °C

Analysis Note

enzyme activity: the optimum temperature is 95-100 °C (the enzyme is stable up to 75 °C), the optimum pH is 6-9 (stable from pH 6-9). Inhibitors: PCMB, Hg+.

Other Notes

An enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptide and proteins; employed in Edman degradation.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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