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17389

Sigma-Aldrich

Pectinase from Aspergillus niger

powder, slightly beige, >1 U/mg

Synonym(s):

Polygalacturonase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

form

powder

specific activity

>1 U/mg

color

slightly beige

storage temp.

2-8°C

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General description

Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants. polygalacturonase is a highly found enzyme among all the pectinolytic enzymes.

Application

Pectinase from Aspergillus niger has been used to hydrolyze pectin layers in
  • activated sludge flocs
  • bamboo powder for degumming process
  • garden cress (Lepidium sativum) seeds to enhance testa permeability, visualized using tetrazolium staining solution

Biochem/physiol Actions

Pectinase found in plants aids in cell wall extension and softening of some plant tissues during maturation and storage. Polygalacturonase is the enzyme that catalyzes the hydrolysis of the polygalacturonic acid chain by addition of water. Pectinase has wide range of industrial applications including, fruit juice extraction and its clarification, scouring of cotton, degumming of plant fibers and waste water treatment. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol galacturonic acid from polygalacturonic acid per minute at pH 4.0 and 50 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility
Fu Jiajia, et al.
Biocatalysis and Biotransformation, 30(1), 27-37 (2012)
Aránzazu Manzano et al.
American journal of botany, 108(9), 1775-1792 (2021-09-16)
Light and gravity are fundamental cues for plant development. Our understanding of the effects of light stimuli on plants in space, without gravity, is key to providing conditions for plants to acclimate to the environment. Here we tested the hypothesis
Microbial pectinolytic enzymes: a review
Jayani RS, et al.
Process Biochemistry (Oxford, United Kingdom), 40(9), 2931-2944 (2005)
Li-Hung Chen et al.
Science advances, 7(19) (2021-05-09)
Effectors are small, secreted proteins that promote pathogen virulence. Although key to microbial infections, unlocking the intrinsic function of effectors remains a challenge. We have previously shown that members of the fungal Avr4 effector family use a carbohydrate-binding module of
Eman Ibrahim et al.
Journal of biochemistry, 169(1), 109-117 (2020-08-19)
Lignocellulosic biomass conversion using cellulases/polygalacturonases is a process that can be progressively influenced by several determinants involved in cellulose microfibril degradation. This article focuses on the kinetics and thermodynamics of thermal inactivation of recombinant Escherichia coli cellulases, cel12B, cel8C and

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