U5128

Urea

ACS reagent, 99.0-100.5%

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352100
• PubChem Substance ID: 24900652
• NACRES: NA.21

Properties

• grade: ACS reagent
• Quality Level: 200
• Assay: ≤0.001% (Heavy Metals by ICP-OES, metals basis); 99.0-100.5%
• form: solid
• impurities: ≤0.01% Insoluble matter
• ign. residue: ≤0.01%
• mp: 132-135 °C (lit.)
• solubility: H₂O: soluble 480 g/L at 20 °C
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 ppm; sulfate (SO₄^2-): ≤0.001%
• cation traces: Fe: ≤0.001%
• functional group: amine
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

Description

General description

Urea is a colourless organic compound, without any odour. It is easily soluble in water.

Application

Urea has been used:

• to adjust the urea nitrogen concentration to a preferred range in blood reservoir for evaluating the dialyzer urea clearance
• to prepare standards to measure urea synthesis
• to prepare standards for blood urea nitrogen assay

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Biochem/physiol Actions

Urea participates in the metabolism of nitrogenous substances. It serves as a nitrogen source, when used in fertilizers. Urea is used in manufacturing chemical compounds. It is widely used in the agricultural industry as a fertilizer as well as an animal feed additive, which is used as a nitrogen supplement in animal husbandry. Urea has adverse effects on seed germination, seedling growth and early plant growth in soil.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves