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Key Documents

C9157

Sigma-Aldrich

Activated charcoal

suitable for cell culture, suitable for plant cell culture

Synonym(s):

Charcoal activated

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About This Item

Empirical Formula (Hill Notation):
C
CAS Number:
Molecular Weight:
12.01
EC Number:
MDL number:
UNSPSC Code:
11101522
PubChem Substance ID:
NACRES:
NA.21

vapor pressure

<0.1 mmHg ( 20 °C)

Quality Level

description

acid-washed with hydrochloric acid

form

powder

autoignition temp.

842 °F

resistivity

1375 μΩ-cm, 20°C (graphite)

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

mp

3550 °C (lit.)

SMILES string

[C]

InChI

1S/C

InChI key

OKTJSMMVPCPJKN-UHFFFAOYSA-N

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General description

Activated charcoal is originally used as an absorbent to remove toxic substances from plants. The use of activated charcoal in culture media gives it a dark colour. It induces absorption of phenolic, cell lysis products, ethylene, methane and many other compounds that are known to be deleterious to the plant tissue. Along with this, activated charcoal also absorbs growth regulators and other media components. In orchid, carrot and tomato tissue culture, activated charcoal induces growth and differentiation.

Application

Activated charcoal has been used:
  • in the pre-treatment of fetal calf serum, which was then used for protein content determination
  • to study its effect on cell viability
  • in a tissue culture medium and to study the availability of 2,4-dichlorophenoxyacetic acid in the same

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Plant Cell and Tissue Culture, 505-505 (2013)
Plant Tissue Culture: Practices and New Experimental Protocols, 41-41 (2007)
Modeling available 2, 4-dichlorophenoxyacetic acid in a tissue culture medium containing activated carbon
Toering A and Pullman G
Plant Cell, Tissue and Organ Culture, 82(2), 179-188 (2005)
G Williamson et al.
Carcinogenesis, 17(11), 2385-2387 (1996-11-01)
It has recently been shown by Hollman et al. (Am. J. Clin. Nutr., 62, 1276-1282) that flavonoid glycosides are preferentially absorbed from dietary onions compared to the flavonoid aglycone. In the light of this, we have compared the bioactivities of
Optimization of a sampling system for recovery and detection of airborne porcine reproductive and respiratory syndrome virus and swine influenza virus
Hermann JR, et al.
Applied and Environmental Microbiology, 72(7), 4811-4818 (2006)

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