Key Documents

View All Documentation

ESGRO Complete Accutase

Name: ESGRO Complete Accutase
Brand: MM

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

Synonym(s):

Stem Cell Tested Accutase

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

eCl@ss:

32160801

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Quality Segment

100

form

liquid

manufacturer/tradename

Chemicon^®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

shipped in

dry ice

storage temp.

-10 to -25°C

Description

General description

ESGRO Complete^™ Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.

Application

Cell Detachment:

1. Thaw Accutase^® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.

Physical form

Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase^® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.

Preparation Note

Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase^® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

cOmplete is a trademark of Roche

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SCR005	A6964	SCR104
Sigma-Aldrich SF006 ESGRO Complete Accutase	Millipore SCR005 Accutase cell detachment solution	Sigma-Aldrich A6964 Accutase^® solution	Sigma-Aldrich SCR104 Accutase^®XL Lyophilized Accutase
description The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.	description A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.	description sterile-filtered, suitable for cell culture	description AccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware
technique(s) cell culture \| stem cell: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) cell culture \| mammalian: suitable
manufacturer/tradename Chemicon^®	manufacturer/tradename Chemicon^®	manufacturer/tradename -	manufacturer/tradename Chemicon^®
Quality Level 100	Quality Level 300	Quality Level 200	Quality Level 300
input sample type: mouse embryonic stem cell(s) sample type induced pluripotent stem cell(s)	input sample type cardiac stem cell(s) sample type neural stem cell(s) sample type pancreatic stem cell(s) sample type: mouse embryonic stem cell(s) sample type: human embryonic stem cell(s) sample type mesenchymal stem cell(s) sample type hematopoietic stem cell(s) sample type induced pluripotent stem cell(s) sample type epithelial cells	input -	input -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
form liquid	form liquid	form liquid	form liquid

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

4195466

4387998

4327024

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.