OP40

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10)

liquid, clone RAS 10, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: RAS 10, monoclonal
• Species reactivity: human, mouse, rat
• Application: —
• Citations: 76

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RAS 10, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Flow Cytometry (2.5 µg/mL); Frozen Sections (2.5 µg/mL); Immunoblotting (0.1-3 µg/mL, chemiluminescent and colorimetric, ; Immunofluorescence (2.5 µg/mL, ; Immunoprecipitation (1 µg/sample); Paraffin Sections (2.5 µg/mL, saponin pre-treatment required))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... KRAS(3845)

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.

Recognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.

This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).

Immunogen

recombinant human p21 Ras

Application

Flow Cytometry (2.5 g/ml)
Frozen Sections (2.5 g/ml)
Immunoblotting (0.1-3 g/ml, chemiluminescent and colorimetric, see application references)
Immunofluorescence (2.5 g/ml, see application references)
Immunoprecipitation (1 g/sample)
Paraffin Sections (2.5 g/ml, saponin pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
SW480 or Y1 cells or breast carcinoma tissue

Other Notes

Rodenhuis, S. et al., 1992. Cancer Res. (Suppl)52, 2665s.
Sidransky, D., et al. 1992. Science256, 102.
Hamer, P.J., et al. 1990. Hybridoma9, 573.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.

The level of expression of p21^ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable