OP15

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

liquid, clone 3B5, Calbiochem^®

• Synonym(s): Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: 3B5, monoclonal
• Species reactivity: human, mouse
• Application: —
• Citations: 38

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3B5, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Frozen Sections (2.5 µg/mL); Immunoblotting (2-3 µg/mL, ; Immunofluorescence (2.5 µg/mL); Immunoprecipitation (1 µg/sample, ; Paraffin Sections (2.5 µg/mL, pressure cooker, heat or trypsin pre-treatment required, )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ERBB2(2064)

Description

General description

Anti-c-ErbB2/c-Neu (Ab-3), mouse monoclonal, clone 3B5, recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells. It is validated for WB, IF, IP, and IHC on frozen and paraffin sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0.Ag14 cells (see application references). Recognizes the ~185 kDa c-Erb2/c-Neu protein.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.

Immunogen

Human

a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu

Application

Frozen Sections (2.5 g/ml)
Immunoblotting (2-3 g/ml, see application references)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g/sample, see application references)
Paraffin Sections (2.5 g/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
HepG2 cells or normal skin

Positive Control
SK-BR-3 cells or breast carcinoma tissue

Other Notes

For paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Mamcs, J.R., et al. 1994. Ann. Surg.219, 332.
Tetu, B., et al. 1994. Cancer73, 2359.
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Bargmann, C.I., et al. 1986. Nature319, 226.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg