MABN92-AF647
Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor™ 647 Conjugate
clone 20A12.1, from mouse, ALEXA FLUOR™ 647
Synonym(s):
Allograft inflammatory factor 1, AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 647
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
20A12.1, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunofluorescence: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AIF1(199)
General description
Allograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.
Application
Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor™ 647 Conjugate is an antibody against AIF1 for use in Immunofluorescence.
The unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.
Quality
Evaluated by Immunofluorescence in human spleen tissue.
Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.
Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.
Target description
~17 kDa observed.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Jamie M Walker et al.
Acta neuropathologica communications, 10(1), 23-23 (2022-02-16)
Clinical symptoms correlate with underlying neurodegenerative changes in the vast majority of people. However, an intriguing group of individuals demonstrate neuropathologic changes consistent with Alzheimer disease (AD) yet remain cognitively normal (termed "resilient"). Previous studies have reported less overall neuronal
Youngmi Kim et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 36(1), 100034-100034 (2023-02-15)
Glioblastoma is a heterogeneous tumor for which effective treatment options are limited and often insufficient. Few studies have examined the intratumoral transcriptional and proteomic heterogeneity of the glioblastoma microenvironment to characterize the spatial distribution of potential molecular and cellular therapeutic
Fumio Takahashi et al.
iScience, 25(5), 104278-104278 (2022-05-17)
Neurodegeneration is a process involving both cell autonomous and non-cell autonomous neuron loss, followed by a collapse of neural networks, but its pathogenesis is poorly understood. We have previously demonstrated that Eomes-positive helper T (Eomes + Th) cells recognizing LINE-1(L1)-derived prototypic
Emmet Huang-Hobbs et al.
Nature, 619(7971), 844-850 (2023-06-29)
The tumour microenvironment plays an essential role in malignancy, and neurons have emerged as a key component of the tumour microenvironment that promotes tumourigenesis across a host of cancers1,2. Recent studies on glioblastoma (GBM) highlight bidirectional signalling between tumours and
Pei Song et al.
Journal of healthcare engineering, 2021, 3048383-3048383 (2021-10-12)
Cardiopulmonary bypass (CPB) is mainly used during cardiac surgeries that treat ischemic, valvular, or congenital heart disease and aortic dissections. The disorders of central nervous system (CNS) that occur after cardiopulmonary bypass are attracting considerable interest. Postoperative neurocognitive disorders (PND)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service