MAB5262
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, mouse monoclonal, RT97
Synonym(s):
Anti-CMT2CC, Anti-NFH
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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product name
Anti-Neurofilament 200 kDa Antibody, clone RT97, clone RT97, Chemicon®, from mouse
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RT97, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
General description
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.
Specificity
The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).
Immunogen
Rat brain protein preparation
Application
Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.
Immunohistochemistry:
1-10 μg/mL
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum
Optimal working dilutions must be determined by end user.
1-10 μg/mL
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Quality
Evaluated by Western Blot on Mouse brain membrane lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.
Target description
200 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Brain
Cultured neurons
Brain
Cultured neurons
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S Hou et al.
Neuroscience, 159(1), 369-379 (2009-01-17)
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The Journal of physiology, 586(21), 5247-5258 (2008-08-30)
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PloS one, 7(2), e30632-e30632 (2012-03-01)
Co-localisation is a widely used measurement in immunohistochemical analysis to determine if fluorescently labelled biological entities, such as cells, proteins or molecules share a same location. However the measurement of co-localisation is challenging due to the complex nature of such
Shuhei Ito et al.
eNeuro, 5(5) (2018-12-19)
Nogo receptor-1 (NgR1) signaling is involved in the limitation of axonal regeneration following spinal cord injury (SCI) through collapsing the growth cone and inhibiting neurite outgrowth. Lateral olfactory tract usher substance (LOTUS), a NgR antagonist, suppresses these pathological conditions. A
SnoN facilitates axonal regeneration after spinal cord injury.
Do, JL; Bonni, A; Tuszynski, MH
Testing null
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