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Key Documents

MAB324

Sigma-Aldrich

Anti-Neuron Specific Enolase Antibody, clone 5E2

clone 5E2, Chemicon®, from mouse

Synonym(s):

NSE

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

5E2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Human gamma neuron specific enolase. Recognizes a 45kD band corresponding to gamma NSE on immunoblots of human brain extracts.

Immunogen

Neuron-specific enolase from human brain.

Application

Anti-Neuron Specific Enolase Antibody, clone 5E2 detects level of Neuron Specific Enolase & has been published & validated for use in ELISA, WB, IC, IH(P).
Immunoblotting

ELISA

Immunohistochemistry. May be used on formalin-fixed, paraffin embedded sections.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Linkage

Replaces: CBL220

Physical form

Format: Purified
Liquid in PBS pH 7.4 containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8 °C for up to 12 months under sterile conditions.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The functional response of U937 macrophage-like cells is modulated by extracellular matrix proteins and mechanical strain.
Loren A Matheson, Geoffrey N Maksym, J Paul Santerre, Rosalind S Labow
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire null
S A Przyborski
Stem cells (Dayton, Ohio), 19(6), 500-504 (2001-11-20)
Embryonal carcinoma cells are pluripotent stem cells derived from germ cell tumors and can be used to study cell differentiation in vitro. This report describes an approach designed to isolate pluripotent stem cells from primary/parent stock cultures of explanted tumor
I Mori et al.
Virology, 287(1), 163-170 (2001-08-16)
Knockout of the interleukin-18 (IL-18) gene predisposed mice to impaired clearance of neurovirulent influenza A virus-infected neurons from the brain. In wild-type mice, IL-18 molecule-producing microglia/macrophages emerged in virally attacked regions as early as day 3 after infection. Microglial transformation
I Mori et al.
Microbes and infection, 2(11), 1329-1334 (2000-10-06)
Neurodegeneration in the brain induced by the WSN strain of influenza A virus was investigated after stereotaxic introduction into the olfactory bulb of C57BL/6 mice. Immunohistochemistry detected WSN virus-infected neurons in the anterior olfactory nucleus as early as day 3
Increased protein levels of serotonin transporter in frontal cortex of patients with Down syndrome.
T Gulesserian, E Engidawork, N Cairns, G Lubec
Neuroscience Letters null

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