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EZML

Mouse Leptin ELISA Kit

measures and quantifies Leptin levels in 10 μL serum or plasma

Synonym(s):

Leptin, Obesity factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Input:
sample type serum
sample type plasma (K2 EDTA)
Assay range:
accuracy: 87-100%, linearity: 100-111%, sensitivity: 0.05 ng/mL, standard curve range: 0.2-30 ng/mL
Species reactivity:
mouse
Storage temp.:
2-8°C
Shipped in:
wet ice
Pricing and availability is not currently available.
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Product Name

Mouse Leptin ELISA, This Mouse Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

Quality Segment

species reactivity

mouse

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 87-100%, linearity: 100-111%, sensitivity: 0.05 ng/mL, standard curve range: 0.2-30 ng/mL

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

UniProt accession no.

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Mouse Leptin ELISA is used to measure and quantify leptin levels in metabolism, endocrine, and neuroscience research. Leptin is a hormone or cytokine derived from adipocytes circulating in the blood. It acts as a regulator of food intake and energy expenditure via the hypothalamus. This hormone plays a role in angiogenesis, lipid and carbohydrate metabolism, and hematopoiesis. Leptin exerts its effects on the cardiovascular, reproductive, and immune systems. The deficiency of leptin in rodents is associated with obesity and insulin resistance.

Application

Mouse Leptin ELISA has been used to determine the levels of leptin in mice serum[1][2] and plasma.[3]

Disclaimer

For research use only. Not for use in diagnostic procedures.

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description

This Mouse Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

description

This Rat Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine research.

description

This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

description

This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.

species reactivity

mouse

species reactivity

rat

species reactivity

human

species reactivity

mouse, rat

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

input

sample type plasma (K2 EDTA)
sample type serum

assay range

accuracy: 87-100%, sensitivity: 0.05 ng/mL, linearity: 100-111%, standard curve range: 0.2-30 ng/mL

assay range

accuracy: 102-122%, linearity: 87-100%, sensitivity: 0.04 ng/mL

assay range

accuracy: 94-114%, intra-assay cv: 1.4-4.9
(Sensitive Assay), intra-assay cv: 2.6-4.6, linearity: 80-105%, linearity: 96-125%
(sensitive assay), sensitivity: 0.125 ng/mL
(sensitive assay), sensitivity: 0.125-20 ng/mL, sensitivity: 0.5 ng/mL, standard curve range: 0.5-100 ng/mL

assay range

accuracy: 80-103%
(Serum: Active Ghrelin), accuracy: 86-102%
(Plasma: Active Ghrelin), accuracy: 92-103%
(Serum: Des-octanoyl Ghrelin), accuracy: 95-107%
(Plasma: Des-octanoyl Ghrelin), inter-assay cv: 1.8-4.5%
intra-assay cv: 0.7-1.3%
(Serum), inter-assay cv: 2.0-3.2%
intra-assay cv: 1.1-1.7%
(Plasma)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

UniProt accession no.

P41159, P41160

UniProt accession no.

P41160, P50596

UniProt accession no.

P41159

UniProt accession no.

Q9EQX0 (mouse), Q9QYH7 (rat), Q9UBU3


pictograms

CorrosionExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

wgk

WGK 3

Storage Class

10 - Combustible liquids



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