71086
KOD Hot Start DNA Polymerase
High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.
Synonym(s):
High fidelity PCR, Hot start PCR, Hot start polymerase, KOD hot start polymerase
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About This Item
UNSPSC Code:
41106314
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
100
200
usage
sufficient for 1000 reactions
sufficient for 200 reactions
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
1.0 U/L
technique(s)
PCR: suitable
input
purified DNA
suitability
suitable for PCR
shipped in
dry ice
storage temp.
−20°C
General description
PCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore′s molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.
Source: Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
Concentration: 1.0 U/l
Nicking activity: None detected
Amplification effiency: Functional PCR; inhibition of activity at 21°C verified
Storage: -20°C
*Manufactured by Toyobo and distributed by EMD. Not available in Japan.
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser¢s own internal research. No other patents rights (such as 5¢ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Source: Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
Concentration: 1.0 U/l
Nicking activity: None detected
Amplification effiency: Functional PCR; inhibition of activity at 21°C verified
Storage: -20°C
*Manufactured by Toyobo and distributed by EMD. Not available in Japan.
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser¢s own internal research. No other patents rights (such as 5¢ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Application
KOD Hot Start DNA Polymerase has been used:
- in the amplification of genomic DNA with high fidelity for sanger sequencing.
- for genomic DNA amplification to screen single-cell clones for homozygous deletion of the targeted exon.
- for the amplification of specific gene families on the genomic DNA isolated from mice lymphoma cells.
Features and Benefits
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
- Long & Accurate PCR: Amplifies genomic DNA up to 12 kbp and plasmid and lambda DNA templates up to 21 kbp with low error rate
- Efficiently amplifies high GC-rich regions
- Antibody-mediated hot start feature reduces mispriming and primer-dimer formation
- Produces blunt end PCR products suitable for cloning
- Convenient room-temperature setup compatible with automation
- Compatible with site-directed mutagenesis protocols
- Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets
Components
•200 U or 5 × 200 UKOD Hot Start DNA Polymerase (1.0 U/µl)
•1.2 ml or 5 × 1.2 ml10X PCR Buffer for KOD Hot Start DNA Polymerase
•1 ml or 5 × 1 ml25 mM MgSO₄
•1 ml or 5 × 1 mldNTP Mix (2 mM each)
•1.2 ml or 5 × 1.2 ml10X PCR Buffer for KOD Hot Start DNA Polymerase
•1 ml or 5 × 1 ml25 mM MgSO₄
•1 ml or 5 × 1 mldNTP Mix (2 mM each)
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
Other Notes
Mizuguchi, H., et al. 1999. J. Biochem. (Tokyo)126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol.289, 835.
Legal Information
Manufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Articles
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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