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Creatine Phosphokinase, Rabbit Skeletal Muscle

Name: Creatine Phosphokinase, Rabbit Skeletal Muscle
Brand: MM

Synonym(s):

Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK

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About This Item

CAS Number:

9001-15-4

EC Number:

2.7.3.2 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.21

MDL number:

MFCD00130876

Specific activity:

≥300 U/mg, 25 °C, 750 U/mg, 37 °C

Biological source:

rabbit skeletal muscle

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Properties

biological source

rabbit skeletal muscle

Quality Segment

200

form

lyophilized

specific activity

≥300 U/mg, 25 °C, 750 U/mg, 37 °C

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO₃, pH 9.0: soluble

foreign activity

ATPase, hexokinase, myokinase <0.001%

shipped in

ambient

storage temp.

−20°C

Description

General description

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Preparation Note

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Other Notes

Bessman, S.P. 1987. Anal. Biochem.161, 519.

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	C3755	238395	P1506
Sigma-Aldrich 2384 Creatine Phosphokinase, Rabbit Skeletal Muscle	Sigma-Aldrich C3755 Creatine Phosphokinase from rabbit muscle	Sigma-Aldrich 238395 Creatine Phosphokinase, Porcine Heart	Sigma-Aldrich P1506 Pyruvate Kinase from rabbit muscle
description -	description Type I, salt-free, lyophilized powder, ≥150 units/mg protein	description Creatine Phosphokinase, Porcine Heart, CAS 9001-15-4, is a native creatine phosphokinase that catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP.	description Type II, ammonium sulfate suspension, 350-600 units/mg protein
biological source rabbit skeletal muscle	biological source -	biological source -	biological source rabbit muscle
specific activity ≥300 U/mg, 25 °C, 750 U/mg, 37 °C	specific activity ≥150 units/mg protein	specific activity ≥300 U/mg	specific activity 350-600 units/mg protein
form lyophilized	form salt-free, lyophilized powder	form lyophilized	form ammonium sulfate suspension
shipped in ambient	shipped in wet ice	shipped in ambient	shipped in -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
solubility 0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO₃, pH 9.0: soluble	solubility 0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow	solubility aqueous buffer: soluble, water: soluble	solubility -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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