1.11723

Casein Digest Soy Lecithin Polysorbate 20 (FCDLP 20) Broth (Base)

NutriSelect^® Prime, according to ISO 16212, ISO 18415, ISO 21149 (TAT Broth Base), powder, pack of 500 g

• Synonym(s): TAT broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: ISO 18415; ISO 21149 (TAT Broth Base); according to ISO 16212
• Quality Level: 200
• description: For sterility tests of viscous or gelatinous materials
• sterility: non-sterile
• form: powder
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Prime
• technique(s): microbiological culture: suitable
• pH: 7.3±± 0.2
• application(s): cosmetics; microbiology; pharmaceutical
• storage temp.: 15-25°C

Description

General description

Casein-peptone lecithin polysorbate broth (base) is a powdered medium recommended for general microbiological analysis. Casein enzymic hydrolysate provides the nitrogen, vitamins, amino acids, and carbon in the broth base. Casein peptone in the medium provides optimal conditions for the spore germination and regeneration of the damaged microorganisms. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.

Application

Casein-peptone lecithin polysorbate broth (base) is recommended for diluting samples of pharmaceutical, cosmetic, and other raw materials or final products for determining the total microbial counts.

Analysis Note

• Appearance (clearness): clear to almost clear
• Appearance (colour): yellowish
• pH-value (25 °C): 7.1 - 7.5
• Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 91%
• Growth (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): 77%
• Growth (Escherichia coli ATCC 8739 (WDCM 00012)): 101%
• Growth (Candida albicans ATCC 10231 (WDCM 00054)): 72%
• Incubation: 45 minutes up to 1 hour at 18 – 27°C (laboratory ambient temperature), aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1