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Key Documents

1.01424

Sigma-Aldrich

May-Grünwald′s eosine-methylene blue solution modified

for microscopy

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About This Item

UNSPSC Code:
41116130
EC Index Number:
200-659-6
NACRES:
NA.05

grade

for microscopy

Quality Level

vapor pressure

128 hPa ( 20 °C)

form

liquid

autoignition temp.

420 °C

composition

C.I. 45380, 0.5 g/L
C.I. 52015, 0.7 g/L
contains CH3OH

expl. lim.

5.5-44 % (v/v) Methanol)

IVD

for in vitro diagnostic use

bp

65 °C/1013 hPa

transition temp

flash point 12 °C

density

0.79 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

The staining method according to May-Grünwald is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue solution and Leishman′s eosin methylene blue solution, this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The 500 ml bottle provides ca. 1500 stainings. The May-Grünwald′s eosin methylene blue solution is an IVD / CE registered / certified product and the 500 ml bottle is sufficient for staining up to 1600 slides in Hellendahl cells. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy (Blood smear): passes test
Erythrocytes: pink to brownish
Nuclei: violet
Eosinophilic granules: red-brown
Neutrophilic granules: light violet
Lymphocyte cytoplasm: blue

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C


Certificates of Analysis (COA)

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