07-689
Anti-EZH2 Antibody
Upstate®, from rabbit
Synonym(s):
KMT6, Enx1, Enhancer of Zeste Homologue 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... EZH2(2146)
Related Categories
General description
Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.
Specificity
EZH2
Immunogen
His-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2)
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.
Quality
routinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells
Target description
95-100kDa
Physical form
Antiserum
Format: Purified
Storage and Stability
stable for 2 years at 2-8°C from date of shipment
Analysis Note
Control
K562 cell lysate, C2C12 cell lysate
K562 cell lysate, C2C12 cell lysate
Other Notes
Please refer to lot specific datasheet.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators.
Landeira, David, et al.
Nature Cell Biology, 12, 618-624 (2010)
Aranzazu Villasante et al.
Theranostics, 6(8), 1119-1130 (2016-06-10)
There is a growing interest in the pivotal role of exosomes in cancer and in their use as biomarkers. However, despite the importance of the microenvironment for cancer initiation and progression, monolayer cultures of tumor cells still represent the main
Satoshi Kawano et al.
PloS one, 11(7), e0158888-e0158888 (2016-07-09)
The catalytic activities of covalent and ATP-dependent chromatin remodeling are central to regulating the conformational state of chromatin and the resultant transcriptional output. The enzymes that catalyze these activities are often contained within multiprotein complexes in nature. Two such multiprotein
EZH2-mediated epigenetic silencing in germinal center B cells contributes to proliferation and lymphomagenesis.
Velichutina, I; Shaknovich, R; Geng, H; Johnson, NA; Gascoyne, RD; Melnick, AM; Elemento, O
Blood null
Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1.
Hong, GM; Bain, LJ
Toxicology and Applied Pharmacology null
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