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Key Documents

05-386

Sigma-Aldrich

Anti-Vinculin Antibody

UPSTATE®, mouse monoclonal, V284

Synonym(s):

Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

product name

Anti-Vinculin Antibody, clone V284, clone V284, Upstate®, from mouse

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V284, monoclonal

species reactivity

human, rat, mouse, rabbit, chicken

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

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This Item
Z250031Z262897Z249963
material

PTFE flat liner, glass bottle, white polypropylene cap, colorless bottle, round bottle

material

PTFE flat liner, amber bottle, glass bottle, round bottle, white polypropylene cap

material

amber bottle, glass bottle, round bottle, vinyl flat liner, white polypropylene cap

material

amber bottle, black plastic cap, glass bottle, round bottle

capacity

8 oz (250 mL)

capacity

8 oz (250 mL)

capacity

8 oz (250 mL)

capacity

8 oz (250 mL)

feature

with cap

feature

with cap

feature

with cap

feature

with cap

joint

Joints threaded neck (24-400)

joint

Joints threaded neck (24-400)

joint

Joints threaded neck (24-400)

joint

Joints threaded neck (24-400)

packaging

pkg of 12 ea

packaging

pkg of 12 ea

packaging

pkg of 12 ea

packaging

pkg of 12 ea

Specificity

Vinculin

Immunogen

Human Vinculin

Application

Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts

Target description

117kDa

Physical form

Format: Purified
PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Protein A chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paul G Leonard et al.
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Butcher, JT; Penrod, AM; Garcia, AJ; Nerem, RM
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Science signaling, 9(431), ra59-ra59 (2016-06-09)
Signal transduction networks coordinate transcriptional programs activated by diverse extracellular stimuli, such as growth factors and cytokines. Cells receive multiple stimuli simultaneously, and mapping how activation of the integrated signaling network affects gene expression is a challenge. We stimulated colon
Temperature dependent specific heat capacity (Cp) of G-actin and talin or talin-vinculin bound to G-actin.
W H Goldmann et al.
Biochemical Society transactions, 20(3), 273S-273S (1992-08-01)
X Wu et al.
The Journal of biological chemistry, 276(32), 30285-30292 (2001-05-31)
The L-type calcium channel is the major calcium influx pathway in vascular smooth muscle and is regulated by integrin ligands, suggesting an important link between extracellular matrix and vascular tone regulation in tissue injury and remodeling. We examined the role

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