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Key Documents

05-384

Sigma-Aldrich

Anti-α-Actinin Antibody, clone AT6/172

clone AT6/172, Upstate®, from mouse

Synonym(s):

Anti-BDPLT15

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AT6/172, monoclonal

species reactivity

rabbit, human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001102

UniProt accession no.

P12814

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACTN1(87)

Specificity

Recognizes α-actinin.

Immunogen

Human α-actinin

Application

Immunoprecipitation is not recommended
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.

Quality

Routinely evaluated in immunoblot on human A431 cells

Target description

100 kDa

Physical form

Format: Purified
Please see product datasheet for lot-specific formulation.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of genes differentially expressed in mouse mammary epithelium transformed by an activated beta-catenin.
Renou, JP; Bierie, B; Miyoshi, K; Cui, Y; Djiane, J; Reichenstein, M; Shani, M; Hennighausen, L
Oncogene null
Xiaoxiong Liu et al.
Journal of the American Heart Association, 7(13) (2018-06-28)
Carboxyl-terminal modulator protein (CTMP) has been implicated in cancer, brain injury, and obesity. However, the role of CTMP in pathological cardiac hypertrophy has not been identified. In this study, decreased expression of CTMP was observed in both human failing hearts
Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function
Fukami, K, et al
Nature, 359, 150-152 (1992)
Jing Zong et al.
Laboratory investigation; a journal of technical methods and pathology, 93(10), 1128-1136 (2013-08-21)
Nucleotide-binding oligomerization domain-2 (NOD2, also designated CARD15), a member of the NOD-leucine-rich repeat (LRR) protein family (also called the CATERPILLAR family), is upregulated in atheroma lesions and has an important role in regulating the intracellular recognition of bacterial components by
Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy.
Bian, Z; Liao, H; Zhang, Y; Wu, Q; Zhou, H; Yang, Z; Fu, J; Wang, T; Yan, L; Shen, D; Li, H; Tang, Q
Testing null
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