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04-1530

Sigma-Aldrich

Anti-MDM2 Antibody, clone 3G9

clone 3G9, from mouse

Synonym(s):

Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Oncoprotein Mdm2, double minute 2, human homolog

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3G9, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_002383.2

UniProt accession no.

Q00987

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

General description

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Specificity

This antibody recognizes MDM2.

Immunogen

Epitope: Unknown
His-tagged recombinant protein corresponding to human MDM2.

Application

Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Ubiquitin & Ubiquitin Metabolism

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.

Target description

~ 75 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhenzhen Liu et al.
Chemical biology & drug design, 85(4), 411-417 (2014-09-18)
In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in
Xiaomu Li et al.
Nature communications, 7, 11740-11740 (2016-06-07)
Mitochondrial metabolism is pivotal for glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells. However, little is known about the molecular machinery that controls the homeostasis of intermediary metabolites in mitochondria. Here we show that the activation of p53 in β-cells, by
Zhuohao Liu et al.
Diabetes, 67(11), 2397-2409 (2018-08-23)
Profound loss and senescence of adipose tissues are hallmarks of advanced age, but the underlying cause and their metabolic consequences remain obscure. Proper function of the murine double minute 2 (MDM2)-p53 axis is known to prevent tumorigenesis and several metabolic
R C Heijkants et al.
Oncogenesis, 7(3), 33-33 (2018-03-30)
Uveal melanoma (UM) is the most frequent ocular cancer in adults, accounting for ~5% of the total melanoma incidence. Although the primary tumor is well treatable, patients frequently develop metastases for which no curative therapy exists. Highly activated protein kinase
Javier Octavio Mejía-Hernández et al.
Cancers, 14(16) (2022-08-27)
Metastatic prostate cancer is a lethal disease in patients incapable of responding to therapeutic interventions. Invasive prostate cancer spread is caused by failure of the normal anti-cancer defense systems that are controlled by the tumour suppressor protein, p53. Upon mutation
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