850500P

CHAPS

Avanti Research^™ - A Croda Brand

• Synonym(s): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₇S
• CAS Number: 75621-03-3
• Molecular Weight: 614.88
• UNSPSC Code: 12161902
• NACRES: NA.25

Properties

• Assay: >99% (TLC)
• form: powder
• mol wt: 614.88 g/mol
• packaging: pkg of 1 × 1 g (850500P-1g)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• technique(s): protein quantification: suitable
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: UMCMPZBLKLEWAF-BCTGSCMUSA-N

Description

General description

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Application

CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.

Packaging

20 mL Clear Glass Screw Cap Vial (850500P-1g)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable