L033000

BTT Activator

0.3 M

• Synonym(s): 5-(Benzylthio)-1H-tetrazole solution
• Empirical Formula (Hill Notation): C₈H₈N₄S
• CAS Number: 21871-47-6
• Molecular Weight: 192.24
• MDL number: MFCD00068731
• UNSPSC Code: 12352100
• PubChem Substance ID: 329817353
• NACRES: NA.21

Properties

• Quality Level: 200
• type: for DNA synthesis
• product line: Proligo Reagents
• storage condition: dry at room temperature
• concentration: 51.9-63.4 g/L (BTT content, titration)
• color: clear
• SMILES string: C(Sc1nnn[nH]1)c2ccccc2
• InChI: 1S/C8H8N4S/c1-2-4-7(5-3-1)6-13-8-9-11-12-10-8/h1-5H,6H2,(H,9,10,11,12)
• InChI key: GXGKKIPUFAHZIZ-UHFFFAOYSA-N

Description

General description

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.BTT Activator 0.3M is 5-(Benzylthio)-1H-tetrazole.

Preparation Note

Acetonitrile / 5-(Benzylthio)-1H-Tetrazole 100 / 6 (v/w)

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H319
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 41.0 °F
• Flash Point(C): 5 °C